1ICX
CRYSTAL STRUCTURE OF PATHOGENESIS-RELATED PROTEIN LLPR10.1A FROM YELLOW LUPINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.3750 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.973, 56.617, 61.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.950 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.053 | 0.127 |
| Total number of observations | 89375 * | |
| Number of reflections | 9446 | |
| <I/σ(I)> | 25.73 | 8.46 |
| Completeness [%] | 99.5 | 96.8 |
| Redundancy | 9.5 * | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | ammonium sulfate, TRIS, sucrose laureate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
