1IB2

CRYSTAL STRUCTURE OF A PUMILIO-HOMOLOGY DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2000-12-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	142.880, 142.880, 111.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.440 - 1.900
R-factor	0.1955 *
Rwork	0.198
R-free	0.24200
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	19.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.400	1.920
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.063 *	0.326 *
Total number of observations	189138 *
Number of reflections	37094 *	3405 *
<I/σ(I)>	21.2	2.3
Completeness [%]	99.6	99.2 *
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	4 *	PEG 6000, Lithium sulphate, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.5 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		100 (mM)
4	1	reservoir	sodium citrate	100 (mM)