1IAS

CYTOPLASMIC DOMAIN OF UNPHOSPHORYLATED TYPE I TGF-BETA RECEPTOR CRYSTALLIZED WITHOUT FKBP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.948
Spacegroup name	C 2 2 21
Unit cell lengths	172.560, 251.617, 140.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.255
Rwork	0.255
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TGF-beta receptor from TGF-beta receptor/FKBP12 structure
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	30.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.312
Number of reflections	59616
<I/σ(I)>	22	3.5
Completeness [%]	89.7	61.1 *
Redundancy	4.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.8	4 *	100 mM MES pH 5.8, 2.4 M ammonium sulfate, 10 % glycerol v/v, 150 mM NaCl, 1 mM NPC-30345, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	100 (mM)
2	1	reservoir	ammonium sulfate	1.95-2.05 (M)