1IAK
HISTOCOMPATIBILITY ANTIGEN I-AK
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.250, 112.250, 99.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iea |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.990 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 * | 0.270 * |
| Total number of observations | 198306 * | |
| Number of reflections | 48139 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 95.7 | 96.6 |
| Redundancy | 4.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.75 | 20 * | 20% PEG 4000, 7% EG, 80MM A.S, 200MM AMM.ACETATE, 50MM NA.ACETATE PH 4.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG | 20 (%) | |
| 3 | 1 | reservoir | ethylene glycol | 7 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 80 (mM) | |
| 5 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 50 (mM) |
