1IAD
REFINED 1.8 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF ASTACIN, A ZINC-ENDOPEPTIDASE FROM THE CRAYFISH ASTACUS ASTACUS L. STRUCTURE DETERMINATION, REFINEMENT, MOLECULAR STRUCTURE AND COMPARISON TO THERMOLYSIN
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.000, 62.000, 98.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.155 |
| Rwork | 0.155 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.739 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.3-0.6 (mM) | 0.005ml |
| 2 | 1 | drop | ammonium sulfate | 1.1 (M) | 0.0015ml |
| 3 | 1 | drop | phosphate | 0.1 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1 (M) |
