1I9T

CRYSTAL STRUCTURE OF THE OXIDIZED RNA TRIPHOSPHATASE DOMAIN OF MOUSE MRNA CAPPING ENZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-28
Detector	MARRESEARCH
Wavelength(s)	1.20374
Spacegroup name	C 2 2 21
Unit cell lengths	62.002, 98.961, 71.627
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.000 - 1.700
R-factor	0.193
Rwork	0.202
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i9s
RMSD bond length	0.010 *
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (BODY))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.155
Total number of observations	145070 *
Number of reflections	24075
<I/σ(I)>	7.8	4.9
Completeness [%]	98.0	96.5
Redundancy	6	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	10 *	15% isopropanol, 50mM sodium cacodylate (pH 6.5), 25mM magnesium chloride, 25mM ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 283K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	isopropanol	15 (%)
3	1	reservoir	sodium cacodylate	50 (mM)
4	1	reservoir		25 (mM)
5	1	reservoir	ammonium sulfate	25 (mM)