1I8I

CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT ROOM TEMPERATURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	2000-04-21
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	111.600, 45.300, 110.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.173
Rwork	0.156
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015 *
RMSD bond angle	3.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MERLOT
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.115
Total number of observations	10214 *
Number of reflections	9684 *
<I/σ(I)>	16.2
Completeness [%]	86.6 *	58.6 *
Redundancy	2.6	2.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	18 *	sodium chloride, MPD, PEG 10K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	4 (mg/ml)
2	1	drop	ADA buffer	40 (mM)	pH7.0
3	1	drop		25 (mM)
4	1	drop	MPD	1 (%(w/v))
5	1	drop	PEG10000	6 (%(w/v))
6	1	reservoir	ADA buffer	200 (mM)	pH7.0
7	1	reservoir	MPD	2-3 (%)
8	1	reservoir	PEG4000	16 (%)