1I7X

BETA-CATENIN/E-CADHERIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	180.800, 134.200, 94.200
Unit cell angles	90.00, 94.30, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.196 *
Rwork	0.196
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Armadillo repeat domain of murine beta-catenin PDB code 2bct.
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.079	0.368
Total number of observations	109516 *
Number of reflections	44525
<I/σ(I)>	8.6
Completeness [%]	98.5	98.6
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	POLYETHYLENEIMINE TRIS-HCL ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEI	0.1 (%)	pH8.5
3	1	reservoir	Tris-HCl	200 (mM)	pH7.5
4	1	reservoir	isopropanol	6 (%(v/v))