1I6H
RNA POLYMERASE II ELONGATION COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.300, 220.700, 191.300 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| Rwork | 0.250 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i3q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.547 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.111 | 0.317 |
| Number of reflections | 96867 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | PEG 6000, AMMONIUM HYDROGEN PHOSPHATE, SODIUM DIHYDROGEN PHOSPHATE, DIOXANE, DTT, pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 100K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | AMMONIUM HYDROGEN PHOSPHATE | ||
| 3 | 1 | 1 | SODIUM DIHYDROGEN PHOSPHATE | ||
| 4 | 1 | 1 | DIOXANE | ||
| 5 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | dioxane | 50 (mM) | |
| 2 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | 390 (mM) | ||
| 4 | 1 | reservoir | PEG6000 | ||
| 5 | 1 | drop | protein | 5-8 (mg) |
