1I5W
A-DNA DECAMER GCGTA(TLN)ACGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 1999-03-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93217 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.140, 43.960, 45.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.400 |
| Rwork | 0.167 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A-DNA decamer |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.460 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.049 | 0.260 |
| Number of reflections | 10950 | |
| <I/σ(I)> | 38.5 | 6.8 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 8.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 295 | MPD, Spermine, KCl, Sodium Cacodylate, pH 6.0. VAPOR DIFFUSION, HANGING DROP at 295 K, temperature 295.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | Sodium Cacodylate | ||
| 3 | 1 | 1 | Spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | decamer solution | 2.8 (mM) | |
| 2 | 1 | drop | MPD | 10 (%) | |
| 3 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 4 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
| 5 | 1 | drop | potassium chloride | 80 (mM) | |
| 6 | 1 | reservoir | MPD | 35 (%(v/v)) |
