1I5N
Crystal structure of the P1 domain of CheA from Salmonella typhimurium
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9802, 0.9300, 0.9805 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.950, 88.720, 115.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.300 - 2.140 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 2.260 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.048 | 0.167 |
| Total number of observations | 206627 * | |
| Number of reflections | 31247 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 98.3 | 91.3 |
| Redundancy | 6.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 * | 285 | POLYETHYLENE GLYCOL 2000 MONOMETHYL ETHER, AMMONIUM SULFATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 25 (mM) | |
| 3 | 1 | reservoir | 25 (mM) | ||
| 4 | 1 | reservoir | EDTA | 3.5 (mM) | |
| 5 | 1 | reservoir | beta-mercaptoethanol | 15 (mM) |
