1I5L
CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.858, 130.438, 70.047 |
| Unit cell angles | 90.00, 115.36, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.750 |
| R-factor | 0.225 * |
| Rwork | 0.225 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i4k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.050 | 0.354 |
| Total number of observations | 70211 * | |
| Number of reflections | 28746 | |
| <I/σ(I)> | 11.2 | 2 |
| Completeness [%] | 97.6 | 97.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 * | 20 * | PEG6000, sodium citrate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | sodium citrate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 12 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.3 |
