1I5I

THE C18S MUTANT OF BOVINE (GAMMA-B)-CRYSTALLIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	1999-06-26
Detector	RIGAKU RAXIS II
Spacegroup name	P 41 21 2
Unit cell lengths	57.850, 57.850, 98.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.223
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gcr
RMSD bond length	0.012
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.074	0.146
Number of reflections	6843
<I/σ(I)>	21.6	9.6
Completeness [%]	97.1	92.8
Redundancy	10	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	chill tube containing protein to 265 K, then bring to 295 K	7	295	100 mM Phosphate, 20 mM DTT, pH 7.0, chill tube containing protein to 265 K, then bring to 295 K