1I4N
CRYSTAL STRUCTURE OF INDOLEGLYCEROL PHOSPHATE SYNTHASE FROM THERMOTOGA MARITIMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8722 |
| Spacegroup name | P 43 |
| Unit cell lengths | 48.930, 48.930, 248.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.169 * |
| Rwork | 0.169 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.625 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.064 | 0.202 |
| Number of reflections | 20875 | |
| <I/σ(I)> | 6.7 | 3.6 |
| Completeness [%] | 99.7 | 96.2 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG 8000, ammonium phosphate, cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | EDTA | 2 (mM) | |
| 3 | 1 | drop | 1,4-DTT | 1 (mM) | |
| 4 | 1 | drop | potassium phosphate | 10 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG8000 | 8-11 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium sulfate | 160-180 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.0 |
