1I4K
CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.842 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.397, 64.563, 129.862 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A seven membered ring of an Sm-like protein from Pyrococcus abyssii. |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 * | 0.353 * |
| Total number of observations | 255898 * | |
| Number of reflections | 63291 | |
| <I/σ(I)> | 14.1 | 3.5 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.04 | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 20 * | PEG 6000, sodium citrate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 12 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.3 |
