1I3Q

RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION

Replaces:  1EN0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.283
Spacegroup name	I 2 2 2
Unit cell lengths	130.700, 224.800, 369.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.100
R-factor	0.229 *
Rwork	0.229
R-free	0.28300
Structure solution method	MIRAS
RMSD bond length	0.008 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.084	0.298
Number of reflections	98315	9073 *
Completeness [%]	99.2	92.7
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	Fu, J., (1999) Cell, 98, 799. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	dioxane	50 (mM)
2	1	reservoir	dithiothreitol	10 (mM)
3	1	reservoir	ammonium phosphate	390 (mM)	or sodium phosphate, pH6.5
4	1	reservoir	PEG6000
5	1	drop	RNA		5-8mg