1I2W
BETA-LACTAMASE FROM BACILLUS LICHENIFORMIS BS3 COMPLEXED WITH CEFOXITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.375 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.344, 106.372, 63.873 |
| Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR ((ONLINE) 3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.030 * | 1.700 |
| High resolution limit [Å] | 1.650 * | 1.650 * |
| Rmerge | 0.072 * | 0.430 * |
| Total number of observations | 143995 * | |
| Number of reflections | 68766 | 26718 * |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 91.8 | 87.1 * |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | PEG 6000, sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 25 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.0 |
