1I21

CRYSTAL STRUCTURE OF YEAST GNA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9324
Spacegroup name	P 21 21 21
Unit cell lengths	64.584, 93.648, 168.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.400
R-factor	0.221 *
Rwork	0.212
R-free	0.28700 *
Structure solution method	MAD
RMSD bond length	0.014 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.078 *	0.135 *
Number of reflections	37568
<I/σ(I)>	6.3	4.8
Completeness [%]	93.8 *	91.6 *
Redundancy	2.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	PEG 600, IMIDAZOLE, MALATE, pH 5.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG600	17-22 (%)
2	1	reservoir	imidazole	0.2 (M)
3	1	reservoir	malate	0.4 (M)
4	1	drop	Tris-HCl	10 (mM)
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	1 (mM)
7	1	drop	protein	10 (mg/ml)