1I10
HUMAN MUSCLE L-LACTATE DEHYDROGENASE M CHAIN, TERNARY COMPLEX WITH NADH AND OXAMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.942, 158.542, 266.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2 * |
| Rwork | 0.197 |
| R-free | 0.25700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9ldt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.815 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 0.195 |
| Number of reflections | 112344 | |
| <I/σ(I)> | 14.18 | 5.36 |
| Completeness [%] | 92.3 | 89 |
| Redundancy | 3.51 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 28 (mg/ml) | |
| 2 | 1 | drop | NADH | 2.5 (mM) | |
| 3 | 1 | drop | sodium oxamate | 1 (mM) | |
| 4 | 1 | drop | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 12 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) |
