1I07

EPS8 SH3 DOMAIN INTERTWINED DIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	300
Collection date	1998-10-09
Spacegroup name	P 1
Unit cell lengths	27.899, 28.517, 37.188
Unit cell angles	107.39, 96.87, 104.56

Refinement procedure

Resolution	26.000 - 1.800
R-factor	0.202 *
Rwork	0.202
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoj
RMSD bond length	0.007
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.930	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.072	0.353
Total number of observations	33871 *
Number of reflections	9088 *
Completeness [%]	94.0	90.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		100 (mM)
2	1	reservoir	sodium formate	100 (mM)
3	1	reservoir	Tris	100 (mM)
4	1	drop	protein	7-10 (mg/ml)