1HZT

CRYSTAL STRUCTURE OF METAL-FREE ISOPENTENYL DIPHOSPHATE:DIMETHYLALLYL DIPHOSPHATE ISOMERASE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	0.97858, 0.97873
Spacegroup name	P 32 2 1
Unit cell lengths	71.400, 71.400, 61.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.370 - 1.450
R-factor	0.192
Rwork	0.192
R-free	0.22400
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Oudjama, Y., (2001) Acta Crystallogr., 57, 287. *
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Oudjama, Y., (2001) Acta Crystallogr., 57, 287. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	PEG2000 MME	10 (%(w/v))
3	1	reservoir	Tris-maleate	100 (mM)
4	1	reservoir	ammonium sulfate	100 (mM)