1HZD
CRYSTAL STRUCTURE OF HUMAN AUH PROTEIN, AN RNA-BINDING HOMOLOGUE OF ENOYL-COA HYDRATASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 1999-11-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.130, 132.070, 80.040 |
Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.207 * |
Rwork | 0.207 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dub |
RMSD bond length | 0.006 |
RMSD bond angle | 21.302 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.046 | 0.123 * |
Total number of observations | 236961 * | |
Number of reflections | 76595 | |
<I/σ(I)> | 13.1 | 2.673 |
Completeness [%] | 96.8 | 91.5 |
Redundancy | 3.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 293 | PEG 8000, calcium acetate, sodium acetate, imidazole, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.5 |
2 | 1 | drop | PEG8000 | 9 (%) | |
3 | 1 | drop | calcium acetate | 100 (mM) | |
4 | 1 | drop | sodium acetate | 100 (mM) | |
5 | 1 | drop | imidazole | 10 (mM) | |
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
7 | 1 | reservoir | PEG8000 | 18 (%) | |
8 | 1 | reservoir | calcium acetate | 200 (mM) |