1HYQ

MIND BACTERIAL CELL DIVISION REGULATOR FROM A. FULGIDUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97900, 0.97920, 0.93930
Spacegroup name	P 43 21 2
Unit cell lengths	90.312, 90.312, 83.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.600
R-factor	0.2058
Rwork	0.206
R-free	0.22520
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.332
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.071	0.281
Number of reflections	10622
<I/σ(I)>	12.2	3.8
Completeness [%]	95.7	98.6
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.6	19 *	2% PEG6000, 100mM TRIS buffer pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	2 (%)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	drop	protein	5 (mg/ml)