1HYG
Crystal structure of MJ0490 gene product, the family of lactate/malate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-02 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 98.213, 98.213, 167.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.510 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.469 |
| Total number of observations | 244121 * | |
| Number of reflections | 20317 | |
| Completeness [%] | 97.5 | 91.2 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 * | Lee, B.I., (2000) Acta Crystallogr. 56, 84. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mM) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | PMSF | 1 (mM) | |
| 6 | 1 | reservoir | detergent | 18 (mM) | |
| 7 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
| 8 | 1 | reservoir | mPEG350 | 10 (%) | |
| 9 | 1 | reservoir | PEG400 | 12 (%) |
