1HY1

CRYSTAL STRUCTURE OF WILD TYPE DUCK DELTA 2 CRYSTALLIN (EYE LENS PROTEIN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1997-07-16
Detector	BRANDEIS - B1
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	93.481, 99.052, 107.177
Unit cell angles	90.00, 102.08, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.206
Rwork	0.208
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	19.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.220
Total number of observations	277694 *
Number of reflections	79045
<I/σ(I)>	15.2
Completeness [%]	93.2	92.1
Redundancy	3.5	3.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	14% PEG 2000 MME, 300mM magnesium chloride, 100mM HEPES, pH 7.5. VAPOR DIFFUSION, HANGING DROP at 298 K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	mPEG2000	14 (%)
3	1	reservoir		300 (mM)
4	1	reservoir	HEPES	100 (mM)