1HXX
OMPF PORIN MUTANT Y106F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | MACSCIENCE |
Wavelength(s) | 1.042 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 118.105, 118.105, 52.565 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.174 * |
Rwork | 0.187 |
R-free | 0.24400 * |
Structure solution method | difference fourier |
RMSD bond length | 0.080 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.065 |
Total number of observations | 71614 * |
Number of reflections | 19807 |
<I/σ(I)> | 7 |
Completeness [%] | 97.9 * |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 2 | PEG2000 | 10.5 (%) |