1HXL

MINIPROTEIN MP-2 (V10A) COMPLEX WITH STREPTAVIDIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	1999-01-13
Detector	MACSCIENCE
Spacegroup name	C 2 2 21
Unit cell lengths	81.989, 82.583, 89.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.290 - 1.800
Rwork	0.215
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MP-2 STREPTAVIDIN COMPLEX
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.076	0.221
Number of reflections	28454
<I/σ(I)>	11.7
Completeness [%]	100.0	99.9
Redundancy	7.86	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	100 MM POTASSIUM ACETATE, AMMONIUM SULFATE, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 298K