1HXK
GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH DEOXYMANNOJIRIMICIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.814, 109.616, 138.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 1.690* |
R-factor | 0.1969 * |
Rwork | 0.197 |
R-free | 0.21560 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hty |
RMSD bond length | 0.006 |
RMSD bond angle | 1.330 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.900 | 1.550 |
High resolution limit [Å] | 1.690 * | 1.690 * |
Rmerge | 0.086 * | 0.186 * |
Number of reflections | 114653 * | |
<I/σ(I)> | 18.3 | 3.3 |
Completeness [%] | 97.8 * | 92.6 * |
Redundancy | 5.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 298 | PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 8.5 (%) | |
2 | 1 | reservoir | MPD | 5 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |