1HX1

CRYSTAL STRUCTURE OF A BAG DOMAIN IN COMPLEX WITH THE HSC70 ATPASE DOMAIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-21
Detector	MARRESEARCH
Wavelength(s)	0.9786, 0.9792, 0.9500
Spacegroup name	C 1 2 1
Unit cell lengths	116.570, 40.780, 129.260
Unit cell angles	90.00, 114.84, 90.00

Refinement procedure

Resolution	14.900 - 1.900
R-factor	0.234 *
Rwork	0.234
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.270
Number of reflections	43748
<I/σ(I)>	21
Completeness [%]	99.5	99.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	PEG3350, Tris, Na-K-tartrate, glycerol, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	PEG3350, Tris, Na-K-tartrate, glycerol, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	sodium potassium tartrate	50-100 (mM)
4	1	reservoir	PEG3350	12-16 (%)
5	1	reservoir	Tris	0.1 (M)
6	1	reservoir	glycerol	25 (%(w/v))