1HWT
STRUCTURE OF A HAP1/DNA COMPLEX REVEALS DRAMATICALLY ASYMMETRIC DNA BINDING BY A HOMODIMERIC PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 108 |
Detector technology | AREA DETECTOR |
Collection date | 1997-06-15 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.600, 85.700, 94.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HAP1_18 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.083 * |
Total number of observations | 41241 * |
Number of reflections | 23274 |
Completeness [%] | 91.6 * |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | 0.3 MM PROTEIN, 0.4 MM DNA DUPLEX, 5% PEG 2000, 100 MM KCL 5 MM MGCL2, 0.1 MM CO(NH3)6CL3, 25 MM MES (PH 5.6), VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KCL | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | [CO(NH3)6]CL3 | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | PEG 2000 | ||
6 | 1 | 2 | PEG 2000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.3 (mM) | |
10 | 1 | reservoir | 10 (mM) | ||
11 | 1 | reservoir | 0.2 (mM) | ||
12 | 1 | reservoir | MES | 50 (mM) | |
2 | 1 | drop | DNA duplex | 0.4 (mM) | |
3 | 1 | drop | PEG2000 | 5 (%) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | 5 (mM) | ||
6 | 1 | drop | 0.1 (mM) | ||
7 | 1 | drop | MES | 25 (mM) | |
8 | 1 | reservoir | PEG2000 | 10 (%) | |
9 | 1 | reservoir | 200 (mM) |