1HVX
BACILLUS STEAROTHERMOPHILUS ALPHA-AMYLASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-01-01 |
Detector | WEISSENBERG |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.550, 93.119, 53.275 |
Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.156 * |
Rwork | 0.154 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.202 |
Number of reflections | 29849 | |
<I/σ(I)> | 21.7 | |
Completeness [%] | 89.0 | 73 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 293 | SODIUM ACETATE, CALCIUM CLORIDE, 2-PROPANOL, ACARBOSE , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 35 (mM) | pH4.6 |
2 | 1 | reservoir | 35 (mM) | ||
3 | 1 | reservoir | 2-propanol | 6.25 (%(v/v)) | |
4 | 1 | reservoir | acarbose | 1.23 (%(w/v)) | |
5 | 1 | drop | protein | 10 (mg/ml) |