1HV4
CRYSTAL STRUCTURE ANALYSIS OF BAR-HEAD GOOSE HEMOGLOBIN (DEOXY FORM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-05-10 |
| Detector | SIEMENS X200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.660, 94.100, 59.230 |
| Unit cell angles | 71.55, 65.10, 83.10 |
Refinement procedure
| Resolution | 36.890 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | IPC |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.890 | 2.730 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.054 * | 0.146 |
| Total number of observations | 57884 * | |
| Number of reflections | 30654 | |
| <I/σ(I)> | 15.1 | 4.4 |
| Completeness [%] | 67.4 | 24 |
| Redundancy | 1.9 | 1.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 18 * | PEG 6000, potassium phosphate , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 5 (%(w/v)) | |
| 3 | 1 | reservoir | PEG6000 | 30 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 50 (mM) |
