1HTQ
Multicopy crystallographic structure of a relaxed glutamine synthetase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 207.720, 257.690, 274.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22300 |
| Structure solution method | Multicopy Refinement |
| Starting model (for MR) | 1hto |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.075 | 0.075 |
| Number of reflections | 566370 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 4000, sodium citrate, sodium chloride, manganese chloride, imidazole buffer , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
