1HTJ
STRUCTURE OF THE RGS-LIKE DOMAIN FROM PDZ-RHOGEF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9717 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 61.610, 61.610, 201.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.223 * |
| Rwork | 0.223 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 | 0.296 |
| Total number of observations | 57934 * | |
| Number of reflections | 12161 | |
| <I/σ(I)> | 12.1 | 4.4 |
| Completeness [%] | 98.7 | 99 * |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 20 * | Ammonium Sulfate, PEG 400, Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 40-42 (%sat) | |
| 5 | 1 | reservoir | PEG400 | 2 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
