1HSL
REFINED 1.89 ANGSTROMS STRUCTURE OF THE HISTIDINE-BINDING PROTEIN COMPLEXED WITH HISTIDINE AND ITS RELATIONSHIP WITH MANY OTHER ACTIVE TRANSPORT(SLASH)CHEMOSENSORY RECEPTORS
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.160, 102.520, 64.980 |
| Unit cell angles | 90.00, 93.56, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.890 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.772 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 1.890 * |
| Number of reflections | 28331 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | PEG8000 | 23 (%) | |
| 3 | 1 | reservoir | Bis-Tris-propane hydrochloride | 0.05 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | 0.2 (M) | ||
| 6 | 1 | drop | PEG8000 | 23 (%) | |
| 7 | 1 | drop | Bis-Tris-propane hydrochloride | 0.05 (%) | |
| 8 | 1 | drop | L-histidine | 0.5 (mM) | |
| 9 | 1 | drop | sodium cacodylate | 10 (mM) |
