1HR6
Yeast Mitochondrial Processing Peptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 1999-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 134.180, 178.500, 202.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 2.500 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.27900 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.081 | 0.541 |
| Total number of observations | 1062534 * | |
| Number of reflections | 167094 | |
| <I/σ(I)> | 23.6 | 2.2 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 6.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 21 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-14 (mg/ml) | |
| 10 | 1 | reservoir | n-dodecylmaltoside | 0.2 (mM) | |
| 11 | 1 | reservoir | sodium azide | 2 (mM) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | glycerol | 30 (%(v/v)) | |
| 4 | 1 | drop | n-dodecylmaltoside | 0.2 (mM) | |
| 5 | 1 | reservoir | PEG10000 | 5-9 (%(w/v)) | |
| 6 | 1 | reservoir | ethylene glycol | 35 (%(v/v)) | |
| 7 | 1 | reservoir | MPD | 6 (%(v/v)) | |
| 8 | 1 | reservoir | sodium citrate | 25 (mM) | |
| 9 | 1 | reservoir | benzamidine | 2 (%(w/v)) |
