1HR2
CRYSTAL STRUCTURE ANALYSIS OF A MUTANT P4-P6 DOMAIN (DELC209) OF TETRAHYMENA THEMOPHILA GROUP I INTRON.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.300, 125.400, 145.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| Rwork | 0.244 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gid |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.510 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.051 | 0.578 |
| Number of reflections | 64900 | |
| <I/σ(I)> | 22.7 | 2.4 |
| Completeness [%] | 98.0 | 99.3 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 30 * | drop is set up using 2:8:5 ratio of solutions a, b and c * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | NaCl | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium cacodylate | 250 (mM) | solution a |
| 10 | 1 | reservoir | MPD | 17 (%(w/v)) | |
| 11 | 1 | reservoir | 170 (mM) | ||
| 2 | 1 | drop | 200 (mM) | solution a | |
| 3 | 1 | drop | spermine | 2.5 (mM) | solution a |
| 4 | 1 | drop | P4-P6 RNA | 4 (mg/ml) | solution b |
| 5 | 1 | drop | potassium cacodylate | 63 (mM) | solution b |
| 6 | 1 | drop | 13 (mM) | solution b | |
| 7 | 1 | drop | cobalt hexammine | 0.16 (mM) | solution b |
| 8 | 1 | drop | EDTA | 0.1 (mM) | solution b |
| 9 | 1 | drop | MPD | 20-25 (%(w/v)) | solution c |
