1HR0

CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93
Spacegroup name	P 41 21 2
Unit cell lengths	399.581, 399.581, 176.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.000 * - 3.200
R-factor	0.218 *
Rwork	0.218
R-free	0.26090
Structure solution method	Difference Fourier using 30S as starting model
Starting model (for MR)	1FJF
RMSD bond length	0.007
RMSD bond angle	28.450 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.140	0.499
Total number of observations	854384 *
Number of reflections	227537 *
<I/σ(I)>	5.1	1.92
Completeness [%]	96.2	92.2
Redundancy	3.75	3.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	277	Wimberly, B.T., (2000) Nature, 407, 327. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	NH4Cl
10	1	2	MPD
11	1	3	initiation factor 1
2	1	1	KCl
3	1	1	magnesium acetate
4	1	1	potasium cacodylate
5	1	1	MPD
6	1	2	NH4Cl
7	1	2	KCl
8	1	2	magnesium acetate
9	1	2	potasium cacodylate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
4	1		2-mercaptoethanol	6 (mM)
5	1		potassium cacodylate	0.1 (M )	or 0.1M MES
6	1	1	MPD	13-17 (%)