1HQZ

Cofilin homology domain of a yeast actin-binding protein ABP1P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	140
Detector technology	IMAGE PLATE
Collection date	1997-11-27
Detector	MARRESEARCH
Wavelength(s)	1.04019
Spacegroup name	C 1 2 1
Unit cell lengths	156.480, 66.680, 125.100
Unit cell angles	90.00, 108.26, 90.00

Refinement procedure

Resolution	20.000 * - 2.100
R-factor	0.213 *
Rwork	0.213
R-free	0.25900
Structure solution method	SIR, PHASED TRANSLATION FUNCTION, MOLECULAR REPLACEMENT
Starting model (for MR)	1cof
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.496 *
Number of reflections	68796 *
<I/σ(I)>	9.9
Completeness [%]	96.6 *	92.6 *
Redundancy	2.3 *	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	PEG 4000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP at 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	22-28 (%)
2	1	reservoir	sodium citrate	0.3 (M)	pH5.6