1HQP

CRYSTAL STRUCTURE OF A TRUNCATED FORM OF PORCINE ODORANT-BINDING PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-02-15
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	89.180, 44.140, 41.240
Unit cell angles	90.00, 107.03, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.204
Rwork	0.204
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3y
RMSD bond length	0.004
RMSD bond angle	16.338 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.047 *	0.107
Total number of observations	14835 *
Number of reflections	5662	1005 *
<I/σ(I)>	31	10.1
Completeness [%]	80.0	64
Redundancy	2.16	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.5	20 *	Ammonium sulfate, Tris, ethylene glycol, pH 7.5, MICRODIALYSIS, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	2	Tris	0.05 (M)
3	1	2	ethylene glycol	3 (%)
4	1	2	ammonium sulfate	2.9 (M)