1HQH
CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-28 |
Spacegroup name | P 32 |
Unit cell lengths | 88.000, 88.000, 112.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.800 |
R-factor | 0.286 |
Rwork | 0.286 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.045 | 0.319 |
Total number of observations | 55538 * | |
Number of reflections | 22632 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 98.9 | 100 |
Redundancy | 2.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 8000, BICINE, manganese chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | Bicine | 50 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
5 | 1 | reservoir | Bicine | 50 (mM) | |
6 | 1 | reservoir | 1 (mM) | ||
7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |