1HQF
CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH N-HYDROXY-L-ARGININE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 32 |
| Unit cell lengths | 88.000, 88.000, 112.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.064 | 0.302 |
| Total number of observations | 31263 * | |
| Number of reflections | 17524 * | 781 * |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 83.7 | 96.7 * |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | Kanyo, Z.F., (1992) J.Mol.Biol., 224, 1175. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Bicine | 50 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 5 | 1 | reservoir | Bicine | 50 (mM) | |
| 6 | 1 | reservoir | 1 (mM) | ||
| 7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |
