1HQA

ALKALINE PHOSPHATASE (H412Q)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	275
Detector technology	AREA DETECTOR
Collection date	1994-08-10
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	I 2 2 2
Unit cell lengths	194.900, 167.000, 76.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.250
R-factor	0.161
Rwork	0.161
RMSD bond length	0.020
RMSD bond angle	23.100 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.900
High resolution limit [Å]	2.200
Rmerge	0.080 *
Total number of observations	170722 *
Number of reflections	58551
Completeness [%]	98.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	30 (mg/ml)
2	1	drop	ammonium sulfate	20 (%sat)
3	1	drop	Tris-HCl	100 (mM)
4	1	drop		10 (mM)
5	1	drop		0.01 (mM)
6	1	reservoir	ammonium sulfate	44 (%sat)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		10 (mM)