1HQ1
STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERSALLY CONSERVED PROTEIN FROM THE SIGNAL RECOGNITION PARTICLE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-05-20 |
Wavelength(s) | 1.039177, 1.039988, 0.999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.555, 78.337, 32.872 |
Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.520 |
R-factor | 0.151 |
Rwork | 0.151 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dul |
RMSD bond length | 0.010 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.070 | 0.490 |
Number of reflections | 51967 | |
<I/σ(I)> | 35.3 | 1.6 |
Completeness [%] | 98.2 | 89.3 |
Redundancy | 10.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | Na-MES, isopropanol, potassium chloride, magnesium chloride, MPD, pH 5.6, EVAPORATION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na-MES | ||
2 | 1 | 1 | isopropanol | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | isopropanol | 10 (%) | |
2 | 1 | reservoir | Na-Mes | 50 (mM) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | 12.5 (mM) |