1HO5
5'-NUCLEOTIDASE (E. COLI) IN COMPLEX WITH ADENOSINE AND PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.900, 75.700, 221.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 2.100 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ush |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.100 |
| Rmerge | 0.054 | 0.298 |
| Number of reflections | 69235 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | Knofel, T., (2001) J.Mol.Biol., 309, 255. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
| 2 | 1 | reservoir | 80 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | adenosine | 5 (mM) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | PEG5000 MME | 16 (%(w/v)) | |
| 7 | 1 | reservoir | ethylenediol | 25 (%(v/v)) |
