1HNI

STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN A COMPLEX WITH THE NONNUCLEOSIDE INHIBITOR ALPHA-APA R 95845 AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	223.300, 69.900, 106.500
Unit cell angles	90.00, 105.40, 90.00

Refinement procedure

Resolution	10.000 * - 2.800
R-factor	0.255
Rwork	0.255
R-free	0.36000 *
RMSD bond length	0.014
RMSD bond angle	25.240 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.800
Number of reflections	30186
Completeness [%]	78.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19-20 (mg/ml)
2	1	reservoir	bis-Tris-propane	50 (mM)
3	1	reservoir	ammonium sulfate	100 (mM)
4	1	reservoir	glycerol	10 (%)
5	1	reservoir	PEG8000	12 (%(w/v))