1HNA

CRYSTAL STRUCTURE OF HUMAN CLASS MU GLUTATHIONE TRANSFERASE GSTM2-2: EFFECTS OF LATTICE PACKING ON CONFORMATIONAL HETEROGENEITY

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	81.190, 83.520, 70.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 1.850
R-factor	0.226
Rwork	0.226
RMSD bond length	0.007
RMSD bond angle	1.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.850 *
Rmerge	0.005 *
Total number of observations	28080 *
Number of reflections	20184 *
Completeness [%]	97.0 *
Redundancy	5.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	130 (mg/ml)	0.18 ml
2	1	drop	GS-DNB	1 (M)	0.0156 ml
3	1	reservoir	Tris-HCl	150 (mM)
4	1	reservoir	BME	5 (mM)
5	1	reservoir	PEG1000	25-30 (%)