1HMP
THE CRYSTAL STRUCTURE OF HUMAN HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE WITH BOUND GMP
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.500, 66.450, 52.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.700 |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.450 * |
| Rmerge | 0.070 * |
| Number of reflections | 16428 * |
| Completeness [%] | 85.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | precipitant |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | precipitant |
| 4 | 1 | reservoir | PEG4000 | 20-24 (%) | precipitant |
| 5 | 1 | reservoir | dithiothreitol | 1-2 (mM) | precipitant |
| 6 | 1 | reservoir | EDTA | 0.5 (mM) | precipitant |
