1HKH
unligated gamma lactamase from an Aureobacterium species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 240.874, 240.874, 240.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.000 - 1.730 |
| Rwork | 0.144 |
| R-free | 0.15800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bro |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.347 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.108 | 0.480 |
| Number of reflections | 119247 | |
| <I/σ(I)> | 24.76 | 5.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.39 * | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 19 * | pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 10 (mM) | pH7.5 |
| 2 | 1 | drop | ammonium sulfate | 100 (%sat) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | ammonium sulfate | 58 (%sat) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
