1HKD
Structure of pea lectin in complex with alpha-methyl-D-glucopyranoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-6 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.793, 61.235, 136.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.950 - 2.090 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ltn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.305 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.420 |
Number of reflections | 30005 | |
<I/σ(I)> | 17.9 | 1.92 |
Completeness [%] | 95.4 | 83.5 |
Redundancy | 2.8 | 2.19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 1.7 M AMMONIUM SULFATE, 10% (V/V) ETHANOL, pH 7.00 |